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KD-Validated Anti-Cyclin dependent kinase 5 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1004
Application
WB, FC, ICC
Uniprot Id
Q00535
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-cyclin dependent kinase 5 antibody (Cat#ABG1004). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-cyclin dependent kinase 5 antibody (Cat#ABG1004, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-cyclin dependent kinase 5 antibody (Cat#ABG1004). Cyclin dependent kinase 5 expression in wild type (WT) and cyclin dependent kinase 5 (CDK5) shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-cyclin dependent kinase 5 antibody (Cat#ABG1004, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of cyclin dependent kinase 5 expression in HepG2 cells using cyclin dependent kinase 5 antibody (Cat#ABG1004, 1:2,000). Green, isotype control; red, cyclin dependent kinase 5.
  • Immunocytochemical staining of HepG2 cells with cyclin dependent kinase 5 antibody (Cat#ABG1004, 1:1,000). Nuclei were stained blue with DAPI; Cyclin dependent kinase 5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1004-100 3 days 100 µL $499.00
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ABG1004-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-Cyclin dependent kinase 5 Rabbit Monoclonal Antibody
Accession (Primary)Q00535
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names24GB5895
Immunogen
A synthesized peptide derived from human Cdk5
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 33 kDa, observed, 33 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:2,000-1:10,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000

Target Information: ( Q00535 )

Gene NameCDK5
Other Gene Names
CDK5; CRK6; Cell division protein kinase 5; EC 2.7.11.22; PSSALRE; TAU protein kinase II catalytic subunit; TPKII catalytic subunit; Tau protein kinase II catalytic subunit; kinase Cdk5
About Gene Symbol
CDK5 (Cell division protein kinase 5) is a protein kinase and signaling regulator involved in cell cycle regulation. It is an important research target in cancer, cell signaling, and therapeutic target research and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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