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KD-Validated Anti-MAP1LC3A Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1011
Application
WB, ICC
Uniprot Id
Q9H492
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-MAP1LC3A antibody (Cat#ABG1011). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-MAP1LC3A antibody (Cat#ABG1011, 1:20,000) and HRP-conjugated goat anti rabbit secondary antibody respectively.
  • Western blotting analysis using anti-MAP1LC3A antibody (Cat.#ABG1011). MAP1LC3A expression in wild type (WT) and MAP1LC3A shRNA knockdown (KD) HeLa cells with 30 µg of Total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-MAP1LC3A antibody (Cat.#ABG1011, 1:20,000) and HRP-conjugated goat anti rabbit secondary antibody respectively.
  • Immunocytochemical staining of C2C12 cells with MAP1LC3A antibody (Cat#ABG1011, 1:1,000). Nuclei were stained blue with DAPI; MAP1LC3A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1011-100 3 days 100 µL $499.00
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ABG1011-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-MAP1LC3A Rabbit Monoclonal Antibody
Accession (Primary)Q9H492
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names23GB 1960
Immunogen
A synthesized peptide derived from human MAP1LC3A
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 14 kDa, observed, 14,16 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:4,000-1:20,000 Immunocytochemistry (IC): 1:1,000

Target Information: ( Q9H492 )

Gene NameMAP1LC3A
Other Gene Names
MAP1LC3A; Microtubule Associated Protein 1 Light Chain 3 Alpha; MAP1BLC3; MAP1ALC3; ATG8E; LC3A; LC3; Microtubule-Associated Proteins 1A/1B Light Chain 3A; Autophagy-Related Ubiquitin-Like Modifier LC3 A; MAP1 Light Chain 3-Like Protein 1; MAP1A/MAP1B Light Chain 3 A; MAP1A/MAP1B LC3 A; Microtubule-Associated Protein 1 Light Chain 3 Alpha; Microtubule-Associated Proteins 1A/1B Light Chain 3; Autophagy-Related Protein LC3 A; MAP1A/1B Light Chain 3 A
About Gene Symbol
MAP1LC3A (Microtubule Associated Protein 1 Light Chain 3 Alpha) is an autophagy regulatory protein involved in autophagy and ubiquitin-proteasome pathway. It is an important research target in cancer, cell signaling, and therapeutic target research and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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