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KD-Validated Anti-Hsp22 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1014
Application
WB, FC, ICC
Uniprot Id
Q9UJY1
Reactivity
Human, Mouse
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-Hsp22 antibody (Cat#ABG1014). Total cell lysates (50 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Hsp22 antibody (Cat#ABG1014, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-Hsp22 antibody (Cat#ABG1014). Hsp22 expression in wild type (WT) and Hsp22 shRNA knockdown (KD) 293T cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Hsp22 antibody (Cat#ABG1014, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of Hsp22 expression in HepG2 cells using Hsp22 antibody (Cat#ABG1014, 1:2,000). Green, isotype control; red, Hsp22.
  • Immunocytochemical staining of HepG2 cells with Hsp22 antibody (Cat#ABG1014, 1:1,000). Nuclei were stained blue with DAPI; Hsp22 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1014-100 3 days 100 µL $499.00
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ABG1014-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-Hsp22 Rabbit Monoclonal Antibody
Accession (Primary)Q9UJY1
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names23GB1980
Immunogen
A synthesized peptide derived from human HSPB8/HSP22
Species Reactivity
Reacts WithHuman, Mouse
Calculated MW
Predicted, 22 kDa , observed, 22 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:500-1:2,500 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000

Target Information: ( Q9UJY1 )

Gene NameHSPB8
Other Gene Names
HSPB8; Heat Shock Protein Family B (Small) Member 8; E2IG1; HSP22; CMT2L; H11; Small Stress Protein-Like Protein HSP22; Heat Shock 27kDa Protein 8; Heat Shock 22kDa Protein 8; Heat Shock Protein Beta-8; E2-Induced Gene 1 Protein; Alpha-Crystallin C Chain; Protein Kinase H11; HSPB8-N1; HSPB8-N2; DHMN2; HMN2A; CRYAC; HspB8; HMN2
About Gene Symbol
HSPB8 (Heat Shock Protein Family B (Small) Member 8) is a molecular chaperone involved in cell signaling and proliferation. It is an important research target in cancer, cell signaling, and therapeutic target research and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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