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KD-Validated Anti-EIF4EBP1 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1015
Application
WB, FC, ICC
Uniprot Id
Q13541
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-EIF4EBP1 antibody (Cat#ABG1015). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-EIF4EBP1 antibody (Cat#ABG1015, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-EIF4EBP1 antibody (Cat#ABG1015). EIF4EBP1 expression in wild type (WT) and EIF4EBP1 shRNA knockdown (KD) Hela cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-EIF4EBP1 antibody (Cat#ABG1015, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of EIF4EBP1 expression in HAP-1 cells using EIF4EBP1 antibody (Cat#ABG1015, 1:2,000). Green, isotype control; red, EIF4EBP1.
  • Immunocytochemical staining of HAP-1 cells with EIF4EBP1 antibody (Cat#ABG1015, 1:1,000). Nuclei were stained blue with DAPI; EIF4EBP1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1015-100 3 days 100 µL $499.00
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ABG1015-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-EIF4EBP1 Rabbit Monoclonal Antibody
Accession (Primary)Q13541
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names23GB 1985
Immunogen
A synthesized peptide derived from human eIF4EBP1
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 13 kDa, observed, 18 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000

Target Information: ( Q13541 )

Gene NameEIF4EBP1
Other Gene Names
EIF4EBP1; Eukaryotic Translation Initiation Factor 4E Binding Protein 1; PHAS-I; 4E-BP1; Phosphorylated Heat- And Acid-Stable Protein Regulated By Insulin 1; Eukaryotic Translation Initiation Factor 4E-Binding Protein 1; EIF4E-Binding Protein 1; 4EBP1; BP-1
About Gene Symbol
EIF4EBP1 (Eukaryotic Translation Initiation Factor 4E Binding Protein 1) is a protein kinase and signaling regulator involved in mTOR/PI3K/Akt signaling and translational regulation. It is an important research target in cancer, cell signaling, and therap
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