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KD-Validated Anti-Diaphanous related formin 1 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1017
Application
WB, FC, ICC
Uniprot Id
O60610
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-Diaphanous related formin 1 antibody (Cat#ABG1017). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Diaphanous related formin 1 antibody (Cat#ABG1017, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-Diaphanous related formin 1 antibody (Cat#ABG1017). Diaphanous related formin 1 expression in wild type (WT) and diaphanous related formin 1 shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Diaphanous related formin 1 antibody (Cat#ABG1017, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of Diaphanous related formin 1 expression in HepG2 cells using Diaphanous related formin 1 antibody (Cat#ABG1017, 1:2,000). Green, isotype control; red, Diaphanous related formin 1.
  • Immunocytochemical staining of HepG2 cells with Diaphanous related formin 1 antibody (Cat#ABG1017, 1:1,000). Nuclei were stained blue with DAPI; Diaphanous related formin 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1017-100 3 days 100 µL $499.00
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ABG1017-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-Diaphanous related formin 1 Rabbit Monoclonal Antibody
Accession (Primary)O60610
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names23GB 1990
Immunogen
A synthesized peptide derived from human DIAPH1
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 141 kDa , observed, 155 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000

Target Information: ( O60610 )

Gene NameDIAPH1
Other Gene Names
DIAPH1; Diaphanous Related Formin 1; HDIA1; LFHL1; Mammalian Diaphanous Related Formin 1; Protein Diaphanous Homolog 1; MDia1; DFNA1; DRF1; Diaphanous (Drosophila, Homolog) 1; Diaphanous Homolog 1 (Drosophila); Diaphanous-Related Formin-1; SCBMS; MDIA1; DIAP1; DIA1
About Gene Symbol
DIAPH1 (Diaphanous Related Formin 1) is a formin actin nucleator involved in cytoskeletal organization. It is an important research target in cell biology, cancer metastasis, and cytoskeletal research and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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