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KD-Validated Anti-CLPX Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1031
Application
WB, FC, ICC
Uniprot Id
O76031
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-CLPX antibody (Cat#ABG1031). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CLPX antibody (Cat#ABG1031, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-CLPX antibody (Cat#ABG1031). CLPX expression in wild-type (WT) and CLPX shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-CLPX antibody (Cat#ABG1031, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of CLPX expression in HepG2 cells using anti-CLPX antibody (Cat#ABG1031, 1:2,000). Green, isotype control; red, CLPX.
  • Immunocytochemical staining of HepG2 cells with CLPX antibody (Cat#ABG1031, 1:1,000). Nuclei were stained blue with DAPI; CLPX was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1031-100 3 days 100 µL $499.00
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ABG1031-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-CLPX Rabbit Monoclonal Antibody
Accession (Primary)O76031
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names24GB5980
Immunogen
A synthesized peptide derived from human CLPX
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 69 kDa, observed, 60 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000

Target Information: ( O76031 )

Gene NameCLPX
Other Gene Names
Caseinolytic Mitochondrial Matrix Peptidase Chaperone Subunit X; ATP-Dependent Clp Protease ATP-Binding Subunit ClpX-Like, Mitochondrial; ClpX (Caseinolytic Protease X, E. Coli) Homolog ; ClpX Caseinolytic Protease X Homolog (E. Coli); Energy-Dependent Regulator Of Proteolysis; ClpX Caseinolytic Peptidase X Homolog; ClpX Caseinolytic Protease X Homolog; EPP2
About Gene Symbol
CLPX (Caseinolytic Mitochondrial Matrix Peptidase Chaperone Subunit X) is a protease enzyme involved in cellular metabolism. It is an important research target in metabolic disease, cancer metabolism, and drug discovery and is widely studied to elucidate
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