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KD-Validated Anti-Frizzled class receptor 9 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1034
Application
WB, ICC
Uniprot Id
O00144
Reactivity
Human
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-frizzled class receptor 9 antibody (Cat#ABG1034). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-frizzled class receptor 9 antibody (Cat#ABG1034, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-frizzled class receptor 9 antibody (Cat#ABG1034). Frizzled class receptor 9 expression in wild type (WT) and frizzled class receptor 9 (FZD9) shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-frizzled class receptor 9 antibody (Cat#ABG1034, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Immunocytochemical staining of HepG2 cells with anti-frizzled class receptor 9 antibody (Cat#ABG1034, 1:1,000). Nuclei were stained blue with DAPI; Frizzled class receptor 9 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1034-100 3 days 100 µL $499.00
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ABG1034-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-Frizzled class receptor 9 Rabbit Monoclonal Antibody
Accession (Primary)O00144
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names24GB5995
Immunogen
A synthesized peptide derived from human Frizzled 9 / CD349
Species Reactivity
Reacts WithHuman
Calculated MW
Predicted, 64 kDa, observed, 50 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Immunocytochemistry (IC): 1:1,000

Target Information: ( O00144 )

Gene NameFZD9
Other Gene Names
FZD9; Frizzled Class Receptor 9; FZD3; CD349; Frizzled 9, Seven Transmembrane Spanning Receptor; Frizzled Family Receptor 9; Frizzled-9; Fz-9; FzE6; HFz9; Frizzled (Drosophila) Homolog 9; Frizzled Homolog 9 (Drosophila); Frizzled Homolog 9; CD349 Antigen
About Gene Symbol
FZD9 (Frizzled Class Receptor 9) is a membrane receptor involved in Wnt/β-catenin signaling and adaptive immunity. It is an important research target in cell signaling, drug discovery, and cancer research and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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