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KD-Validated Anti-MHC class 1 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1048
Application
WB, FC, ICC
Uniprot Id
P04439
Reactivity
Human
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-MHC class 1 antibody (Cat#ABG1048). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-MHC class 1 antibody (Cat#ABG1048, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-MHC class 1 antibody (Cat#ABG1048). MHC class 1 expression in wild type (WT) and HLA-A shRNA knockdown (KD) HT-1080 cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-MHC class 1 antibody (Cat#ABG1048, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of MHC class 1 expression in HeLa cells using MHC class 1 antibody (Cat#ABG1048, 1:2,000). Green, isotype control; red, MHC class 1.
  • Immunocytochemical staining of HAP-1 cells with anti-MHC class 1 antibody (Cat#ABG1048, 1:1,000). Nuclei were stained blue with DAPI; MHC class 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:High. Scale bar, 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1048-100 3 days 100 µL $499.00
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ABG1048-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-MHC class 1 Rabbit Monoclonal Antibody
Accession (Primary)P04439
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names24GB6040
Immunogen
A synthesized peptide derived from human MHC class 1
Species Reactivity
Reacts WithHuman
Calculated MW
Predicted, 41 kDa, observed, 41 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000

Target Information: ( P04439 )

Gene NameHLA-A
Other Gene Names
HLA-A; Major Histocompatibility Complex, Class I, A; HLA Class I Histocompatibility Antigen, A Alpha Chain; HLAA; HLA Class I Histocompatibility Antigen, A-1 Alpha Chain; MHC Class I Antigen HLA-A Heavy Chain; Leukocyte Antigen Class I-A; Human Leukocyte Antigen A
About Gene Symbol
HLA-A (Major Histocompatibility Complex, Class I, A) is a membrane receptor involved in adaptive immunity. It is an important research target in cell signaling, drug discovery, and cancer research and is widely studied to elucidate cellular mechanisms and
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