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KD-Validated Anti-RPS6KA1 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1049
Application
WB, FC, ICC
Uniprot Id
Q15418
Reactivity
Human
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-RPS6KA1 antibody (Cat#ABG1049). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-RPS6KA1 antibody (Cat#ABG1049, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-RPS6KA1 antibody (Cat#ABG1049). RPS6KA1 expression in wild type (WT) and RPS6KA1 shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-RPS6KA1 antibody (Cat#ABG1049, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of RPS6KA1 expression in HeLa cells using RPS6KA1 antibody (Cat#ABG1049, 1:2,000). Green, isotype control; red, RPS6KA1.
  • Immunocytochemical staining of Hela cells with RPS6KA1 antibody (Cat#ABG1049, 1:1,000). Nuclei were stained blue with DAPI; RPS6KA1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1049-100 3 days 100 µL $499.00
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ABG1049-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-RPS6KA1 Rabbit Monoclonal Antibody
Accession (Primary)Q15418
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names23GB 2080
Immunogen
A synthesized peptide derived from human RSK1 p90
Species Reactivity
Reacts WithHuman
Calculated MW
Predicted, 83 kDa, observed, 83 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000

Target Information: ( Q15418 )

Gene NameRPS6KA1
Other Gene Names
RPS6KA1; Ribosomal Protein S6 Kinase A1; RSK1; MAPKAPK1; HU-1; MAP Kinase-Activated Protein Kinase 1a; 90 KDa Ribosomal Protein S6 Kinase 1; Ribosomal Protein S6 Kinase Alpha-1; MAPK-Activated Protein Kinase 1a; Ribosomal S6 Kinase 1; MAPKAP Kinase 1a; EC 2.7.11.1; MAPKAPK-1a; MAPKAPK1A; P90-RSK 1; P90RSK1; P90Rsk; P90S6K; RSK-1; DJ590P13.1 (Ribosomal Protein S6 Kinase, 90kD, Polypeptide 1); Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1; Ribosomal Protein S6 Kinase, 90kD, Polypeptide 1; S6K-Alpha 1; S6K-Alpha-1; EC 2.7.11; P90RSK; RSK
About Gene Symbol
RPS6KA1 (Ribosomal Protein S6 Kinase A1) is a mitogen-activated protein kinase involved in MAPK/ERK signaling and translational regulation. It is an important research target in cancer, cell signaling, and therapeutic target research and is widely studied
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