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KD-Validated Anti-Crystallin alpha B Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1056
Application
WB, FC, ICC
Uniprot Id
P02511
Reactivity
Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-crystallin alpha B antibody (Cat#ABG1056). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-crystallin alpha B antibody (Cat#ABG1056, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-crystallin α B antibody (Cat#ABG1056). Crystallin α B expression in wild type (WT) and crystallin α B (CRYAB) shRNA knockdown (KD) H9c2 cells with 20 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-crystallin α B antibody (Cat#ABG1056, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of Crystallin alpha B expression in H9c2 cells using crystallin alpha B antibody (Cat#ABG1056, 1:2,000). Green, isotype control; red, crystallin alpha B.
  • Immunocytochemical staining of HT-1080 cells with crystallin alpha B antibody (Cat#ABG1056, 1:1,000). Nuclei were stained blue with DAPI; Crystallin alpha B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1056-100 3 days 100 µL $499.00
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ABG1056-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-Crystallin alpha B Rabbit Monoclonal Antibody
Accession (Primary)P02511
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names24GB6070
Immunogen
A synthesized peptide derived from human CRYAB
Species Reactivity
Reacts WithRat
Calculated MW
Predicted, 20 kDa, observed, 18 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000

Target Information: ( P02511 )

Gene NameCRYAB
Other Gene Names
CRYAB; Crystallin Alpha B; HSPB5; CRYA2; Renal Carcinoma Antigen NY-REN-27; Heat Shock Protein Beta-5; Rosenthal Fiber Component; Alpha-Crystallin B Chain; Epididymis Secretory Protein Li 101; Heat-Shock 20 KD Like-Protein; Crystallin, Alpha B; Alpha(B)-Crystallin; HEL-S-101; CTRCT16; CMD1II; CTPP2; HspB5; MFM2
About Gene Symbol
CRYAB (Crystallin Alpha B) is a molecular chaperone involved in adaptive immunity. It is an important research target in cancer research and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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