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KD-Validated Anti-Gli3 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1063
Application
WB, FC, ICC
Uniprot Id
P10071
Reactivity
Human
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-gli3 antibody (Cat#ABG1063). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-gli3 antibody (Cat#ABG1063, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-gli3 antibody (Cat#ABG1063). Gli3 expression in wild-type (WT) and GLI3 shRNA knockdown (KD) HT-1080 cells with 30 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-gli3 antibody (Cat#ABG1063, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of gli3 expression in HepG2 cells using anti-gli3 antibody (Cat#ABG1063, 1:2,000). Green, isotype control; red, gli3.
  • Immunocytochemical staining of HepG2 cells with anti-gli3 antibody (Cat#ABG1063, 1:1,000). Nuclei were stained blue with DAPI; Gli3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1063-100 3 days 100 µL $499.00
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ABG1063-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-Gli3 Rabbit Monoclonal Antibody
Accession (Primary)P10071
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names24GB6105
Immunogen
A synthesized peptide derived from human Gli3
Species Reactivity
Reacts WithHuman
Calculated MW
Predicted, 170 kDa, observed, 190 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000

Target Information: ( P10071 )

Gene NameGLI3
Other Gene Names
GLI Family Zinc Finger 3; PAP-A; PAPA1; PPDIV; PAPA; PAPB; ACLS; Glioma-Associated Oncogene Family Zinc Finger 3; GLI-Kruppel Family Member GLI3;Transcriptional Activator GLI3; Zinc Finger Protein GLI3; Oncogene GLI3; GLI3-190; GLI3FL; GCPS; PHS; Greig Cephalopolysyndactyly Syndrome; GLI3 Full-Length Protein; GLI3 Form Of 190 KDa; DNA-Binding Protein
About Gene Symbol
GLI3 (GLI Family Zinc Finger 3) is a transcription factor involved in Hedgehog signaling. It is an important research target in cancer research and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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