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KD-Validated Anti-RAC3 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1077
Application
WB, FC, ICC
Uniprot Id
P60763
Reactivity
Human, Mouse
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-RAC3 antibody (Cat#ABG1077). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PSMB10 antibody (Cat#ABG1077, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-RAC3 antibody (Cat#ABG1077). RAC3 expression in wild type (WT) and RAC3 shRNA knockdown (KD) Hela cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-RAC3 antibody (Cat#ABG1077, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of RAC3 expression in HAP-1 cells using RAC3 antibody (Cat#ABG1077, 1:2,000). Green, isotype control; red, RAC3.
  • Immunocytochemical staining of HAP-1 cells with RAC3 antibody (Cat#ABG1077, 1:1,000). Nuclei were stained blue with DAPI; RAC3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1077-100 3 days 100 µL $499.00
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ABG1077-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-RAC3 Rabbit Monoclonal Antibody
Accession (Primary)P60763
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names23GB 2175
Immunogen
A synthesized peptide derived from human RAC3
Species Reactivity
Reacts WithHuman, Mouse
Calculated MW
Predicted, 21 kDa, observed, 21 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000

Target Information: ( P60763 )

Gene NameRAC3
Other Gene Names
RAC3; Rac Family Small GTPase 3; Ras-Related C3 Botulinum Toxin Substrate 3 (Rho Family, Small GTP Binding Protein Rac3); Ras-Related C3 Botulinum Toxin Substrate 3; P21-Rac3; Rho Family, Small GTP Binding Protein Rac3; EC 3.6.5.2
About Gene Symbol
RAC3 (Rac Family Small GTPase 3) is a small GTPase involved in cell signaling and proliferation. It is an important research target in cancer, cell signaling, and therapeutic target research and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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