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KD-Validated Anti-Pancreatic proteinase 1 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1080
Application
WB, FC, ICC
Uniprot Id
P00492
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-pancreatic proteinase 1 antibody (Cat#ABG1080). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-pancreatic proteinase 1 antibody (Cat#ABG1080, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-pancreatic proteinase 1 antibody (Cat#ABG1080). Pancreatic proteinase 1 expression in wild-type (WT) and pancreatic proteinase 1 (HPRT1) shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-pancreatic proteinase 1 antibody (Cat#ABG1080, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of pancreatic proteinase 1 expression in HepG2 cells using pancreatic proteinase 1 antibody (Cat#ABG1080, 1:2,000). Green, isotype control; red, pancreatic proteinase 1.
  • Immunocytochemical staining of HepG2 cells with anti-pancreatic proteinase 1 antibody (Cat#ABG1080, 1:1,000). Nuclei were stained blue with DAPI; Pancreatic proteinase 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1080-100 3 days 100 µL $499.00
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ABG1080-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-Pancreatic proteinase 1 Rabbit Monoclonal Antibody
Accession (Primary)P00492
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names24GB6185
Immunogen
A synthesized peptide derived from human HPRT
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 25 kDa, observed, 25 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000

Target Information: ( P00492 )

Gene NameHPRT1
Other Gene Names
HPRT1; Hypoxanthine Phosphoribosyltransferase 1; HGPRT; HPRT; Hypoxanthine Guanine Phosphoribosyl Transferase; Hypoxanthine-Guanine Phosphoribosyltransferase; EC 2.4.2.8; HGPRTase; Hypoxanthine-Guanine Phosphoribosyltransferase 1; Testicular Tissue Protein Li 89; Lesch-Nyhan Syndrome
About Gene Symbol
HPRT1 (Hypoxanthine Phosphoribosyltransferase 1) is a protein kinase and signaling regulator involved in cell signaling and proliferation. It is an important research target in cancer, cell signaling, and therapeutic target research and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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