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KD-Validated Anti-NME1 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1081
Application
WB, FC, ICC
Uniprot Id
P15531
Reactivity
Human
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-NME1 antibody (Cat#ABG1081). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NME1 antibody (Cat#ABG1081, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-NME1 antibody (Cat#ABG1081). NME1 expression in wild type (WT) and NME1 shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-NME1 antibody (Cat#ABG1081, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of NME1 expression in HepG2 cells using NME1 antibody (Cat#ABG1081, 1:2,000). Green, isotype control; red, NME1.
  • Immunocytochemical staining of HepG2 cells with NME1 antibody (Cat#ABG1081, 1:1,000). Nuclei were stained blue with DAPI; NME1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1081-100 3 days 100 µL $499.00
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ABG1081-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-NME1 Rabbit Monoclonal Antibody
Accession (Primary)P15531
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names23GB 2195
Immunogen
A synthesized peptide derived from human NM23
Species Reactivity
Reacts WithHuman
Calculated MW
Predicted, 17 kDa , observed, 17 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000

Target Information: ( P15531 )

Gene NameNME1
Other Gene Names
NME1; NME/NM23 Nucleoside Diphosphate Kinase 1; NM23-H1; NDPKA; NM23; Non-Metastatic Cells 1, Protein (NM23A) Expressed In; Tumor Metastatic Process-Associated Protein; Metastasis Inhibition Factor Nm23; Nucleoside Diphosphate Kinase A; Granzyme A-Activated DNase; NDP Kinase A; EC 2.7.4.6; GAAD; Epididymis Secretory Sperm Binding Protein; NDPK-A; NDK A; NDKA; AWD; NBS; NB
About Gene Symbol
NME1 (NME/NM23 Nucleoside Diphosphate Kinase 1) is a protein kinase and signaling regulator involved in cell migration and invasion. It is an important research target in cancer research and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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