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KD-Validated Anti-Janus kinase 1 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1086
Application
WB, ICC
Uniprot Id
P23458
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-Janus kinase 1 antibody (Cat#ABG1086). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Janus kinase 1 antibody (Cat#ABG1086, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-Janus kinase 1 antibody (Cat#ABG1086). Janus kinase 1 expression in wild type (WT) and Janus kinase 1 shRNA knockdown (KD) 293T cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Janus kinase 1 antibody (Cat#ABG1086, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Immunocytochemical staining of HepG2 cells with Janus kinase 1 antibody (Cat#ABG1086, 1:1,000). Nuclei were stained blue with DAPI; Janus kinase 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1086-100 3 days 100 µL $499.00
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ABG1086-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-Janus kinase 1 Rabbit Monoclonal Antibody
Accession (Primary)P23458
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names23GB 2245
Immunogen
A synthesized peptide derived from human JAK1
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 133 kDa , observed, 133 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Immunocytochemistry (IC): 1:100-1:1,000

Target Information: ( P23458 )

Gene NameJAK1
Other Gene Names
JAK1; Janus Kinase 1; JAK1A; JAK1B; JTK3; Tyrosine-Protein Kinase JAK1; EC 2.7.10.2; EC 2.7.10; AIIDE; JAK-1
About Gene Symbol
JAK1 (Janus Kinase 1) is a receptor tyrosine kinase involved in cell signaling and proliferation. It is an important research target in cancer, cell signaling, and therapeutic target research and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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