KD-Validated Anti-Transcription elongation factor A1 Rabbit Monoclonal Antibody

Western blotting analysis using anti-Transcription elongation factor A1 antibody (Cat#ABG1101). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Transcription elongation factor A1 antibody (Cat#ABG1101, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.

Western blotting analysis using anti-Transcription elongation factor A1 antibody (Cat#ABG1101). Transcription elongation factor A1 expression in wild type (WT) and transcription elongation factor A1 shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Transcription elongation factor A1 antibody (Cat#ABG1101, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.

Flow cytometric analysis of Transcription elongation factor A1 expression in HepG2 cells using Transcription elongation factor A1 antibody (Cat#ABG1101, 1:2,000). Green, isotype control; red, Transcription elongation factor A1.

Immunocytochemical staining of HepG2 cells with Transcription elongation factor A1 antibody (Cat#ABG1101, 1:1,000). Nuclei were stained blue with DAPI; Transcription elongation factor A1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 µm.