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KD-Validated Anti-RACK1 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1104
Application
WB, FC, ICC
Uniprot Id
P63244
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-RACK1 antibody (Cat#ABG1104). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-RACK1 antibody (Cat#ABG1104, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-RACK1 antibody (Cat#ABG1104). RACK1 expression in wild-type (WT) and RACK1 shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-RACK1 antibody (Cat#ABG1104, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of RACK1 expression in HepG2 cells using RACK1 antibody (Cat#ABG1104, 1:2,000). Green, isotype control; red, RACK1.
  • Immunocytochemical staining of HepG2 cells with RACK1 antibody (Cat#ABG1104, 1:1,000). Nuclei were stained blue with DAPI; RACK1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1104-100 3 days 100 µL $499.00
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ABG1104-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-RACK1 Rabbit Monoclonal Antibody
Accession (Primary)P63244
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names23GB 2355
Immunogen
A synthesized peptide derived from human RACK1
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 35 kDa , observed, 35 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000

Target Information: ( P63244 )

Gene NameRACK1
Other Gene Names
RACK1; Receptor For Activated C Kinase 1; GNB2L1; H12.3; Guanine Nucleotide Binding Protein (G Protein), Beta Polypeptide 2-Like 1; Guanine Nucleotide-Binding Protein Subunit Beta-Like Protein 12.3; Guanine Nucleotide-Binding Protein Subunit Beta-2-Like 1; Cell Proliferation-Inducing Gene 21 Protein ; Receptor Of Activated Protein C Kinase 1; Small Ribosomal Subunit Protein RACK1; Human Lung Cancer Oncogene 7 Protein ; Gnb2-Rs1; HLC-7; Protein Homologous To Chicken B Complex Protein, Guanine Nucleotide Binding; Guanine Nucleotide Binding Protein Beta Polypeptide 2-Like 1; Receptor Of Activated Protein Kinase C 1; Receptor For Activated C Kinase; Proliferation-Inducing Gene 21; Lung Cancer Oncogene 7; GNB2-RS1; PIG21
About Gene Symbol
RACK1 (Receptor For Activated C Kinase 1) is a membrane receptor involved in translational regulation. It is an important research target in cancer research and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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