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KD-Validated Anti-KLF4 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1129
Application
WB, FC, ICC
Uniprot Id
O43474
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-KLF4 antibody (Cat#ABG1129). KLF4 expression in wild type (WT) and KLF4 knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-KLF4 antibody (Cat#ABG1129, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of KLF4 expression in HepG2 cells using KLF4 antibody (Cat#ABG1129, 1:2,000). Green, isotype control; red, KLF4.
  • Immunocytochemical staining of C2C12 cells with anti-KLF4 antibody (Cat#ABG1129, 1:1,000). Nuclei were stained blue with DAPI; KLF4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 µm.
  • Western blotting analysis using anti-KLF4 antibody (Cat#ABG1129). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-KLF4 antibody (Cat#ABG1129, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1129-100 3 days 100 µL $499.00
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ABG1129-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-KLF4 Rabbit Monoclonal Antibody
Accession (Primary)O43474
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names25GB2120
Immunogen
A synthesized peptide derived from human KLF4
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 55 kDa; observed, 54 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western blotting (WB): 1:1,000-1:5,000Flow Cytometry (FCM): 1:2,000Immunocytochemistry (IC): 1:100-1:1,000

Target Information: ( O43474 )

Gene NameKLF4
Other Gene Names
KLF Transcription Factor 4; GKLF; EZF; Epithelial Zinc Finger Protein EZF; Gut-Enriched Krueppel-Like Factor; Kruppel-Like Factor 4 (Gut); Gut Kruppel-Like Factor; Krueppel-Like Factor 4; Kruppel Like Factor 4; Endothelial Kruppel-Like Zinc Finger Protein
About Gene Symbol
KLF4 (KLF Transcription Factor 4) is a transcription factor involved in angiogenesis. It is an important research target in gene regulation, development, and cancer research and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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