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KD-Validated Anti-MAP3K7 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1134
Application
WB, FC, ICC
Uniprot Id
O43318
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-MAP3K7 antibody (Cat#ABG1134). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-MAP3K7 antibody (Cat#ABG1134, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-MAP3K7 antibody (Cat#ABG1134). MAP3K7 expression in wild type (WT) and MAP3K7 shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-MAP3K7 antibody (Cat#ABG1134, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of MAP3K7 expression in HeLa cells using MAP3K7 antibody (Cat#ABG1134, 1:2,000). Green, isotype control; red, MAP3K7.
  • Immunocytochemical staining of Hela cells with MAP3K7 antibody (Cat#ABG1134, 1:1,000). Nuclei were stained blue with DAPI; MAP3K7 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Very low. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1134-100 3 days 100 µL $499.00
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ABG1134-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-MAP3K7 Rabbit Monoclonal Antibody
Accession (Primary)O43318
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names23GB 2395
Immunogen
A synthesized peptide derived from human TAK1
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 67 kDa , observed, 75 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000

Target Information: ( O43318 )

Gene NameMAP3K7
Other Gene Names
MAP3K7; Mitogen-Activated Protein Kinase Kinase Kinase 7; MEKK7; TAK1; Transforming Growth Factor-Beta-Activated Kinase 1; TGF-Beta Activated Kinase 1; EC 2.7.11.25; TGF-Beta-Activated Kinase 1; EC 2.7.11; TGF1a; CSCF; FMD2
About Gene Symbol
MAP3K7 (Mitogen-Activated Protein Kinase Kinase Kinase 7) is a mitogen-activated protein kinase involved in MAPK/ERK signaling and TGF-β signaling. It is an important research target in cancer, cell signaling, and therapeutic target research and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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