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KD-Validated Anti-PKM2 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1139
Application
WB, FC, ICC
Uniprot Id
P14618
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-PKM2 antibody (Cat#ABG1139). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PKM2 antibody (Cat#ABG1139, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-PKM2 antibody (Cat#ABG1139). PKM2 expression in wild-type (WT) and PKM knockdown (KD) HT-1080 cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-PKM2 antibody (Cat#ABG1139, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of PKM2 expression in HT-1080 cells using PKM2 antibody (Cat#ABG1139, 1:2,000). Green, isotype control; red, PKM2.
  • Immunocytochemical staining of C2C12 cells with anti-PKM2 antibody (Cat#ABG1139, 1:1,000). Nuclei were stained blue with DAPI; PKM2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1139-100 3 days 100 µL $499.00
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ABG1139-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-PKM2 Rabbit Monoclonal Antibody
Accession (Primary)P14618
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names25GB2170
Immunogen
A synthesized peptide derived from human PKM2
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 58 kDa; observed, 58 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western blotting (WB): 1:1,000-1:5,000Flow Cytometry (FCM): 1:2,000Immunocytochemistry (IC): 1:100-1:1,000

Target Information: ( P14618 )

Gene NamePKM
Other Gene Names
Pyruvate Kinase M1/2; THBP1; OIP3; PK3PKM2; Cytosolic Thyroid Hormone-Binding Protein; ThyroidHormone-Binding Protein 1; Pyruvate Kinase Muscle Isozyme; Threonine-Protein KinasePKM2; Tyrosine-Protein Kinase PKM2; Pyruvate Kinase, Muscle; Pyruvate Kinase PKM; Pyruvate Kinase 2/3; Tumor M2-PK; EC 2.7.1.40 48; CTHBP; P58; Thyroid Hormone-Binding Protein, Cytosolic; Epididymis Secretory Protein Li; Pyruvate Kinase Isozymes M1/M2; OPA-Interacting Protein 3; Opa-Interacting Protein 3; PK, Muscle Type; EC 2.7.11.1; EC 2.7.10.2; HEL-S-30; OIP-3; TCB; PK2
About Gene Symbol
PKM (Pyruvate Kinase M1/2) is a receptor tyrosine kinase involved in cell signaling and proliferation. It is an important research target in cancer research and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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