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KD-Validated Anti-ACSL4 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1150
Application
WB, FC, ICC
Uniprot Id
O60488
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-ACSL4 antibody (Cat#ABG1150). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ACSL4 antibody (Cat#ABG1150, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-ACSL4 antibody (Cat#ABG1150). ACSL4 expression in wild type (WT) and ACSL4 shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-ACSL4 antibody (Cat#ABG1150, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of ACSL4 expression in HT-1080 cells using ACSL4 antibody (Cat#ABG1150, 1:2,000). Green, isotype control; red, ACSL4.
  • Immunocytochemical staining of HT-1080 cells with ACSL4 antibody (Cat#ABG1150, 1:1,000). Nuclei were stained blue with DAPI; ACSL4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1150-100 3 days 100 µL $499.00
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ABG1150-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-ACSL4 Rabbit Monoclonal Antibody
Accession (Primary)O60488
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names23GB2960
Immunogen
A synthesized peptide derived from ACSL4
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 79 kDa, observed, 73 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000

Target Information: ( O60488 )

Gene NameACSL4
Other Gene Names
ACSL4; Acyl-CoA Synthetase Long Chain Family Member 4; LACS4; ACS4; FACL4; Fatty-Acid-Coenzyme A Ligase, Long-Chain 4; Long-Chain Fatty-Acid-Coenzyme A Ligase 4; Long-Chain-Fatty-Acid--CoA Ligase 4; Long-Chain Acyl-CoA Synthetase 4; Lignoceroyl-CoA Synthase; Arachidonate--CoA Ligase; EC 6.2.1.3; MRX63; MRX68; Mental Retardation, X-Linked 63; Mental Retardation, X-Linked 68; Acyl-CoA Synthetase 4; EC 6.2.1.15; XLID63; LACS 4
About Gene Symbol
ACSL4 (Acyl-CoA Synthetase Long Chain Family Member 4) is a biosynthetic enzyme involved in cell signaling and proliferation. It is an important research target in cancer, cell signaling, and therapeutic target research and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.

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Research Use Only
For research use only. Not intended for diagnostic or therapeutic procedures.