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KD-Validated Anti-CD44 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1164
Application
WB, FC, ICC
Uniprot Id
P16070
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-CD44 antibody (Cat#ABG1164). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CD44 antibody (Cat#ABG1164, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-CD44 antibody (Cat#ABG1164). CD44 expression in wild type (WT) and CD44 shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-CD44 antibody (Cat#ABG1164, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of CD44 expression in HT-1080 cells using CD44 antibody (Cat#ABG1164, 1:2,000). Green, isotype control; red, CD44.
  • Immunocytochemical staining of HT-1080 cells with CD44 antibody (Cat#ABG1164, 1:1,000). Nuclei were stained blue with DAPI; CD44 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1164-100 3 days 100 µL $499.00
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ABG1164-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-CD44 Rabbit Monoclonal Antibody
Accession (Primary)P16070
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names23GB 3055
Immunogen
A synthesized peptide derived from human CD44
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 82 kDa , observed, 81 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000

Target Information: ( P16070 )

Gene NameCD44
Other Gene Names
CD44 Molecule (Indian Blood Group); HUTCH-I; HCELL; CSPG8; MC56; Pgp1; MDU2; MDU3; MIC4; IN; Hematopoietic Cell E- And L-Selectin Ligand; GP90 Lymphocyte Homing/Adhesion Receptor; Chondroitin Sulfate Proteoglycan 8; Extracellular Matrix Receptor III; Homing Cell Adhesion Molecule; Heparan Sulfate Proteoglycan; Phagocytic Glycoprotein 1; Phagocyte Glycoprotein 1; Hyaluronate Receptor; In(Lu) Related-P80; Hermes Antigen; CD44 Antigen; Hermes-1; ECMR-III; HUTCH-1; ECM-III; Epican; PGP-1; CD44R; CDw44; H-CAM; LHR; CD44 Antigen (Homing Function And Indian Blood Group System); Homing Function And Indian Blood Group System; Extracellular Matrix Receptor-III; Cell Surface Glycoprotein CD44; Indian Blood Group Antigen; Phagocytic Glycoprotein I; Soluble CD44; CDW44; PGP-I
About Gene Symbol
CD44 (CD44 Molecule (Indian Blood Group)) is a membrane receptor involved in adaptive immunity. It is an important research target in cell signaling, drug discovery, and cancer research and is widely studied to elucidate cellular mechanisms and identify t
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