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KD-Validated Anti-Catenin alpha 1 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1177
Application
WB, FC, ICC
Uniprot Id
P35221
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-Catenin alpha 1 antibody (Cat#ABG1177). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Catenin alpha 1 antibody (Cat#ABG1177, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-Catenin alpha 1 antibody (Cat#ABG1177). Catenin alpha 1 expression in wild type (WT) and Catenin alpha 1 shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Catenin alpha 1 antibody (Cat#ABG1177, 1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of Catenin alpha 1 expression in C2C12 cells using Catenin alpha 1 antibody (Cat#ABG1177, 1:2,000). Green, isotype control; red, Catenin alpha 1.
  • Immunocytochemical staining of C2C12 cells with Catenin alpha 1 antibody (Cat#ABG1177, 1:1,000). Nuclei were stained blue with DAPI; Catenin alpha 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1177-100 3 days 100 µL $499.00
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ABG1177-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-Catenin alpha 1 Rabbit Monoclonal Antibody
Accession (Primary)P35221
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names23GB3335
Immunogen
A synthesized peptide derived from human alpha 1 Catenin
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 100 kDa , observed, 100 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000

Target Information: ( P35221 )

Gene NameCTNNA1
Other Gene Names
CTNNA1; Catenin Alpha 1; CAP102; Renal Carcinoma Antigen NY-REN-13; Alpha-E-Catenin; Catenin Alpha-1; Catenin (Cadherin-Associated Protein), Alpha 1 (102kD); Catenin (Cadherin-Associated Protein), Alpha 1, 102kDa; Epididymis Secretory Sperm Binding Protein; Cadherin-Associated Protein; Alpha E-Catenin; MDBS2; MDPT2
About Gene Symbol
CTNNA1 (Catenin Alpha 1) is a cell junction/adhesion protein involved in adaptive immunity. It is an important research target in cancer research and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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