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KD-Validated Anti-Caspase 3 p12 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1180
Application
WB, FC, ICC
Uniprot Id
P42574
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-Caspase 3 p12 antibody (Cat#ABG1180). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Caspase 3 p12 antibody (Cat#ABG1180, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-Caspase 3 p12 antibody (Cat#ABG1180). Total cell lysates (30 µg) from H9c2 and HAP-1 cells untreated or treated with staurosporine (1 µM, 3hr) were loaded and separated by SDS-PAGE. The blot was incubated with anti-Caspase 3 p12 antibody (Cat#ABG1180, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-Caspase-3 p12 antibody (Cat#ABG1180). Caspase-3 p12 expression in wild type (WT) and Caspase-3 p12 shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Caspase-3 p12 antibody (Cat#ABG1180, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of Caspase 3 p12 expression in C2C12 cells using Caspase 3 p12 antibody (Cat#ABG1180, 1:2,000). Green, isotype control; red, Caspase 3 p12.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1180-100 3 days 100 µL $499.00
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ABG1180-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-Caspase 3 p12 Rabbit Monoclonal Antibody
Accession (Primary)P42574
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names23GB3310
Immunogen
A synthesized peptide derived from human Caspase-3 p12
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 32 kDa , observed, 12,29,33 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000

Target Information: ( P42574 )

Gene NameCASP3
Other Gene Names
CASP3; Caspase 3; CPP32; Apopain; CPP32B; Caspase 3, Apoptosis-Related Cysteine Peptidase; Caspase 3, Apoptosis-Related Cysteine Protease; SREBP Cleavage Activity 1; Cysteine Protease CPP32; Protein Yama; EC 3.4.22.56; Caspase-3; CASP-3; CPP-32; SCA-1; PARP Cleavage Protease; Procaspase3; EC 3.4.22; APOPAIN; Yama; YAMA
About Gene Symbol
CASP3 (Caspase 3) is a cysteine protease (caspase) involved in apoptosis. It is an important research target in metabolic disease, cancer metabolism, and drug discovery and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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