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KD-Validated Anti-Cyclin A1/A2 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1183
Application
WB, FC, ICC
Uniprot Id
P20248/ P78396
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-Cyclin A1/A2 antibody (Cat#ABG1183). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Cyclin A1/A2 antibody (Cat#ABG1183, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-Cyclin A1 antibody (Cat#ABG1183). Cyclin A1 expression in wild type (WT) and Cyclin A1 shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Cyclin A1 antibody (Cat#ABG1183, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of Cyclin A1/A2 expression in HAP-1 cells using Cyclin A1/A2 antibody (Cat#ABG1183, 1:2,000). Green, isotype control; red, Cyclin A1/A2.
  • Immunocytochemical staining of HAP-1 cells with Cyclin A1/A2 antibody (Cat#ABG1183, 1:1,000). Nuclei were stained blue with DAPI; Cyclin A1/A2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1183-100 3 days 100 µL $499.00
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ABG1183-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-Cyclin A1/A2 Rabbit Monoclonal Antibody
Accession (Primary)P20248/ P78396
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names23GB3525
Immunogen
A synthesized peptide derived from human Cyclin A1/A2
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 52 kDa ; Observed, 52 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western blotting (WB): 1:1,000-1:5,000 Flow cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000

Target Information: ( P20248/ P78396 )

Gene NameCCNA1/2
Other Gene Names
CCNA2; Cyclin A2; CCN1; CCNA; Cyclin-A2; Cyclin-A; Cyclin A; CCNA1; Cyclin A1; CT146; Cyclin-A1; Testicular Tissue Protein Li 34
About Gene Symbol
CCNA1/2 (Cyclin A2) is a protein kinase and signaling regulator involved in cell cycle regulation. It is an important research target in cancer, cell signaling, and therapeutic target research and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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