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KD-Validated Anti-CDC25C Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1184
Application
WB, FC, ICC
Uniprot Id
P30307
Reactivity
Human
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-CDC25C antibody (Cat#ABG1184). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CDC25C antibody (Cat#ABG1184, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-CDC25C antibody (Cat#ABG1184). CDC25C expression in wild type (WT) and CDC25C shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-CDC25C antibody (Cat#ABG1184, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of CDC25C expression in HeLa cells using CDC25C antibody (Cat#ABG1184, 1:2,000). Green, isotype control; red, CDC25C.
  • Immunocytochemical staining of Hela cells with CDC25C antibody (Cat#ABG1184, 1:1,000). Nuclei were stained blue with DAPI; CDC25C was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1184-100 3 days 100 µL $499.00
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ABG1184-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-CDC25C Rabbit Monoclonal Antibody
Accession (Primary)P30307
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names23GB3430
Immunogen
A synthesized peptide derived from human Cdc25C
Species Reactivity
Reacts WithHuman
Calculated MW
Predicted, 53 kDa , observed, 60 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000

Target Information: ( P30307 )

Gene NameCDC25C
Other Gene Names
CDC25C; Cell Division Cycle 25C; M-Phase Inducer Phosphatase 3; PPP1R60; Protein Phosphatase 1, Regulatory Subunit 60; EC 3.1.3.48; CDC25; Dual Specificity Phosphatase CDC25C; Dual Specificity Phosphatase Cdc25C; Phosphotyrosine Phosphatase; CDC25 Homolog C (S. Pombe); Mitosis Inducer CDC25; CDC25 Homolog C
About Gene Symbol
CDC25C (Cell Division Cycle 25C) is a protein phosphatase involved in cell cycle regulation. It is an important research target in metabolic disease, cancer metabolism, and drug discovery and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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