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KD-Validated Anti-CDC16 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1185
Application
WB, FC, ICC
Uniprot Id
Q13042
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-CDC16 antibody (Cat#ABG1185). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CDC16 antibody (Cat#ABG1185, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-CDC16 antibody (Cat#ABG1185). CDC16 expression in wild type (WT) and CDC16 shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-CDC16 antibody (Cat#ABG1185, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of CDC16 expression in HeLa cells using CDC16 antibody (Cat#ABG1185, 1:2,000). Green, isotype control; red, CDC16.
  • Immunocytochemical staining of Hela cells with CDC16 antibody (Cat#ABG1185, 1:1,000). Nuclei were stained blue with DAPI; CDC16 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1185-100 3 days 100 µL $499.00
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ABG1185-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-CDC16 Rabbit Monoclonal Antibody
Accession (Primary)Q13042
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names23GB 3415
Immunogen
A synthesized peptide derived from human Cdc16/APC6
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 72 kDa , observed, 72 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000

Target Information: ( Q13042 )

Gene NameCDC16
Other Gene Names
CDC16; Cell Division Cycle 16; ANAPC6; APC6; CUT9; Cell Division Cycle Protein 16 Homolog; Anaphase-Promoting Complex, Subunit 6; Cyclosome Subunit 6; CDC16Hs; CDC16 (Cell Division Cycle 16, S. Cerevisiae, Homolog); CDC16 Cell Division Cycle 16 Homolog (S. Cerevisiae); Cell Division Cycle 16 Homolog (S. Cerevisiae); Anaphase-Promoting Complex Subunit 6; Cell Division Cycle 16 Homolog; CDC16 Homolog
About Gene Symbol
CDC16 (Cell Division Cycle 16) is a protein kinase and signaling regulator involved in cell signaling and proliferation. It is an important research target in cancer, cell signaling, and therapeutic target research and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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