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KD-Validated Anti-CDC40 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1186
Application
WB, FC, ICC
Uniprot Id
O60508
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-CDC40 antibody (Cat#ABG1186). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CDC40 antibody (Cat#ABG1186, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-CDC40 antibody (Cat#ABG1186). CDC40 expression in wild type (WT) and CDC40 shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-CDC40 antibody (Cat#ABG1186, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of CDC40 expression in HT-1080 cells using CDC40 antibody (Cat#ABG1186, 1:2,000). Green, isotype control; red, CDC40.
  • Immunocytochemical staining of HT-1080 cells with CDC40 antibody (Cat#ABG1186, 1:1,000). Nuclei were stained blue with DAPI; CDC40 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1186-100 3 days 100 µL $499.00
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ABG1186-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-CDC40 Rabbit Monoclonal Antibody
Accession (Primary)O60508
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names23GB3445
Immunogen
A synthesized peptide derived from human CDC40
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 66 kDa , observed, 74 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000

Target Information: ( O60508 )

Gene NameCDC40
Other Gene Names
CDC40; Cell Division Cycle 40; PRPF17; PRP17; EHB3; Cell Division Cycle 40 Homolog; Pre-MRNA-Processing Factor 17; EH-Binding Protein 3; PRP17 Homolog; FLJ10564; HPRP17; Cell Division Cycle 40 Homolog (S. Cerevisiae); Cell Division Cycle 40 Homolog (Yeast); Pre-MRNA Splicing Factor 17; PCH15; Ehb3
About Gene Symbol
CDC40 (Cell Division Cycle 40) is a protein kinase and signaling regulator involved in translational regulation. It is an important research target in cancer, cell signaling, and therapeutic target research and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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