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KD-Validated Anti-Carbonic anhydrase II Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1195
Application
WB, FC, ICC
Uniprot Id
P00918
Reactivity
Human
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-Carbonic anhydrase II antibody (Cat#ABG1195). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Carbonic anhydrase II antibody (Cat#ABG1195, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-Carbonic anhydrase II antibody (Cat#ABG1195). Carbonic anhydrase II expression in wild type (WT) and Carbonic anhydrase II shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Carbonic anhydrase II antibody (Cat#ABG1195, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of Carbonic anhydrase II expression in HeLa cells using Carbonic anhydrase II antibody (Cat#ABG1195, 1:2,000). Green, isotype control; red, Carbonic anhydrase II.
  • Immunocytochemical staining of Hela cells with Carbonic anhydrase II antibody (Cat#ABG1195, 1:1,000). Nuclei were stained blue with DAPI; Carbonic anhydrase II was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1195-100 3 days 100 µL $499.00
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ABG1195-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-Carbonic anhydrase II Rabbit Monoclonal Antibody
Accession (Primary)P00918
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names23GB 3300
Immunogen
A synthesized peptide derived from human Carbonic anhydrase 2
Species Reactivity
Reacts WithHuman
Calculated MW
Predicted, 29 kDa , observed, 29 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000

Target Information: ( P00918 )

Gene NameCA2
Other Gene Names
CA2; Carbonic Anhydrase 2; CA-II; Carbonic Anhydrase II; CAII; Carbonate Dehydratase II; Cyanamide Hydratase CA2; Carbonic Anhydrase C; EC 4.2.1.1; Car2; CAC; Epididymis Secretory Protein Li 282; Epididymis Luminal Protein 76; Carbonic Anhydrase B; Carbonic Dehydratase; EC 4.2.1.69; HEL-S-282; HEL-76; CAR2
About Gene Symbol
CA2 (Carbonic Anhydrase 2) is a metabolic enzyme involved in cellular metabolism. It is an important research target in metabolic disease, cancer metabolism, and drug discovery and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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