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KD-Validated Anti-DDX5 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1197
Application
WB, FC, ICC
Uniprot Id
P17844
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-DDX5 antibody (Cat#ABG1197). DDX5 expression in wild type (WT) and DDX5 shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-DDX5 antibody (Cat#ABG1197, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of DDX5 expression in HepG2 cells using DDX5 antibody (Cat#ABG1197, 1:2,000). Green, isotype control; red, DDX5.
  • Immunocytochemical staining of HepG2 cells with DDX5 antibody (Cat#ABG1197, 1:1,000). Nuclei were stained blue with DAPI; DDX5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 µm.
  • Western blotting analysis using anti-DDX5 antibody (Cat#ABG1197). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-DDX5 antibody (Cat#ABG1197, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1197-100 3 days 100 µL $499.00
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ABG1197-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-DDX5 Rabbit Monoclonal Antibody
Accession (Primary)P17844
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names23GB3550
Immunogen
A synthesized peptide derived from human DDX5
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 69 kDa , observed, 69 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000

Target Information: ( P17844 )

Gene NameDDX5
Other Gene Names
DDX5; DEAD-Box Helicase 5; G17P1; HLR1; P68; DEAD/H (Asp-Glu-Ala-Asp/His); Box Polypeptide 5 (RNA Helicase, 68kD); DEAD (Asp-Glu-Ala-Asp) Box; Polypeptide 5; Probable ATP-Dependent RNA Helicase DDX5; DEAD (Asp-Glu-Ala-Asp) Box Helicase 5; DEAD Box Protein 5; RNA Helicase P68; ATP-Dependent RNA Helicase DDX5; EC 3.6.4.13; DEAD Box-5; EC 3.6.1; HUMP68; HELR
About Gene Symbol
DDX5 (DEAD-Box Helicase 5) is a metabolic enzyme involved in cellular metabolism. It is an important research target in metabolic disease, cancer metabolism, and drug discovery and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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