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KD-Validated Anti-ADP ribosylation factor 5 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1233
Application
WB, FC, ICC
Uniprot Id
P84085
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-ADP ribosylation factor 5 antibody (Cat#ABG1233). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ADP ribosylation factor 5 antibody (Cat#ABG1233, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-ADP ribosylation factor 5 antibody (Cat#ABG1233). ADP ribosylation factor 5 expression in wild type (WT) and ADP ribosylation factor 5 shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-ADP ribosylation factor 5 antibody (Cat#ABG1233, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of ADP ribosylation factor 5 expression in HepG2 cells using ADP ribosylation factor 5 antibody (Cat#ABG1233, 1:2,000). Green, isotype control; red, ADP ribosylation factor 5.
  • Immunocytochemical staining of HepG2 cells with ADP ribosylation factor 5 antibody (Cat#ABG1233, 1:1,000). Nuclei were stained blue with DAPI; ADP ribosylation factor 5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1233-100 3 days 100 µL $499.00
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ABG1233-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-ADP ribosylation factor 5 Rabbit Monoclonal Antibody
Accession (Primary)P84085
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names23GB3635
Immunogen
A synthesized peptide derived from human ARF5
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 21 kDa , observed, 18 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000

Target Information: ( P84085 )

Gene NameARF5
Other Gene Names
ARF5; ADP Ribosylation Factor 5; ADP-Ribosylation Factor 5
About Gene Symbol
ARF5 (ADP Ribosylation Factor 5) is a protein kinase and signaling regulator involved in cell signaling and proliferation. It is an important research target in cancer, cell signaling, and therapeutic target research and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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