KD-Validated Anti-Prolyl 4-hydroxylase subunit beta Rabbit Monoclonal Antibody

Western blotting analysis using anti-Prolyl 4-hydroxylase subunit beta antibody (Cat#ABG1234). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Prolyl 4-hydroxylase subunit beta antibody (Cat#ABG1234, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.

Western blotting analysis using anti-Prolyl 4-hydroxylase subunit beta antibody (Cat#ABG1234). Prolyl 4-hydroxylase subunit beta expression in wild type (WT) and prolyl 4-hydroxylase subunit beta shRNA knockdown (KD) HT1080 cells with 30 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Prolyl 4-hydroxylase subunit beta antibody (Cat#ABG1234, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.

Flow cytometric analysis of Prolyl 4-hydroxylase subunit beta expression in C2C12 cells using Prolyl 4-hydroxylase subunit beta antibody (Cat#ABG1234, 1:2,000). Green, isotype control; red, Prolyl 4-hydroxylase subunit beta.

Immunocytochemical staining of HepG2 cells with anti-prolyl 4-hydroxylase subunit beta antibody (Cat#ABG1234, 1:1,000). Nuclei were stained blue with DAPI; Prolyl 4-hydroxylase subunit beta was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.