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KD-Validated Anti-MUC4 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1237
Application
WB, FC, ICC
Uniprot Id
Q99102
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-MUC4 antibody (Cat#ABG1237). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-MUC4 antibody (Cat#ABG1237, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-MUC4 antibody (Cat#ABG1237). MUC4 expression in wild-type (WT) and MUC4 knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-MUC4 antibody (Cat#ABG1237, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of MUC4 expression in HeLa cells using MUC4 antibody (Cat#ABG1237, 1:2,000). Green, isotype control; red, MUC4.
  • Immunocytochemical staining of HeLa cells with anti-MUC4 antibody (Cat#ABG1237, 1:1000). Nuclei were stained blue with DAPI; MUC4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1237-100 3 days 100 µL $499.00
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ABG1237-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-MUC4 Rabbit Monoclonal Antibody
Accession (Primary)Q99102
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names25GB2595
Immunogen
A synthesized peptide derived from human MUC4
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 232 kDa; observed, 120 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western blotting (WB): 1:1,000-1:5,000Flow Cytometry (FCM): 1:2,000Immunocytochemistry (IC): 1:100-1:1,000

Target Information: ( Q99102 )

Gene NameMUC4
Other Gene Names
Mucin 4, Cell Surface Associated; Ascites Sialoglycoprotein; MUC-4; ASGP; Pancreatic Adenocarcinoma Mucin; Mucin 4, Tracheobronchial; Tracheobronchial Mucin; Testis Mucin; Mucin-4; HSA276359
About Gene Symbol
MUC4 (Mucin 4, Cell Surface Associated) is a protein kinase and signaling regulator involved in cell signaling and proliferation. It is an important research target in cancer research and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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