KD-Validated Anti-Helicase like transcription factor Rabbit Monoclonal Antibody

Western blotting analysis using anti-Helicase like transcription factor antibody (Cat#ABG1239). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Helicase like transcription factor antibody (Cat#ABG1239, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.

Western blotting analysis using anti-Helicase like transcription factor antibody (Cat#ABG1239). Helicase like transcription factor expression in wild type (WT) and helicase like transcription factor shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Helicase like transcription factor antibody (Cat#ABG1239, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.

Immunocytochemical staining of HepG2 cells with Helicase like transcription factor antibody (Cat#ABG1239, 1:1,000). Nuclei were stained blue with DAPI; Helicase like transcription factor was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 µm.