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KD-Validated Anti-Hsp90 beta Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1242
Application
WB, FC, ICC
Uniprot Id
P08238
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-Hsp90 beta antibody (Cat#ABG1242). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Hsp90 beta antibody (Cat#ABG1242, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-Hsp90 beta antibody (Cat#ABG1242). Hsp90 beta expression in wild type (WT) and Hsp90 beta shRNA knockdown (KD) 293T cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Hsp90 beta antibody (Cat#ABG1242, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of HSP90AB1 expression in HepG2 cells using HSP90AB1 antibody (Cat#ABG1242, 1:2,000). Green, isotype control; red, HSP90AB1.
  • Immunocytochemical staining of HepG2 cells with Hsp90 beta antibody (Cat#ABG1242, 1:1,000). Nuclei were stained blue with DAPI; Hsp90 beta was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1242-100 3 days 100 µL $499.00
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ABG1242-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-Hsp90 beta Rabbit Monoclonal Antibody
Accession (Primary)P08238
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names23GB 2770
Immunogen
A synthesized peptide derived from human Hsp90 beta
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 83 kDa , observed, 90 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000

Target Information: ( P08238 )

Gene NameHSP90AB1
Other Gene Names
HSP90AB1; Heat Shock Protein 90 Alpha Family Class B Member 1; HSPC2; HSPCB; Heat Shock Protein 90kDa Alpha (Cytosolic), Class B Member 1; Heat Shock 90kD Protein 1, Beta; Heat Shock Protein HSP 90-Beta; Heat Shock 84 KDa; HSP90B; HSP84; Heat Shock Protein 90kDa Alpha Family Class B Member 1; Heat Shock 90kDa Protein 1, Beta; Heat Shock Protein 90 KDa; HSP90-Beta; D6S182; HSP 90; HSP 84
About Gene Symbol
HSP90AB1 (Heat Shock Protein 90 Alpha Family Class B Member 1) is a molecular chaperone involved in cell signaling and proliferation. It is an important research target in cancer, cell signaling, and therapeutic target research and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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