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KD-Validated Anti-Hsc70 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1244
Application
WB, ICC
Uniprot Id
P11142
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-Hsc70 antibody (Cat#ABG1244). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Hsc70 antibody (Cat#ABG1244, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-Hsc70 antibody (Cat#ABG1244). Hsc70 expression in wild type (WT) and Hsc70 shRNA knockdown (KD) 293T cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Hsc70 antibody (Cat#ABG1244, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Immunocytochemical staining of HepG2 cells with anti-Hsc70 antibody (Cat#ABG1244, 1:1,000). Nuclei were stained blue with DAPI; Hsc70 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1244-100 3 days 100 µL $499.00
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ABG1244-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-Hsc70 Rabbit Monoclonal Antibody
Accession (Primary)P11142
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names23GB2675
Immunogen
A synthesized peptide derived from human Hsc70
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 71 kDa , observed, 70 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Immunocytochemistry (IC): 1:1,000

Target Information: ( P11142 )

Gene NameHSPA8
Other Gene Names
HSPA8; Heat Shock Protein Family A (Hsp70) Member 8; HSC70; HSP73; HSPA10; HSC71; Lipopolysaccharide-Associated Protein 1; Heat Shock Cognate 71 KDa Protein; Heat Shock 70kDa Protein 8; LPS-Associated Protein 1; LAP-1; Epididymis Secretory Sperm Binding Protein Li 72p; N-Myristoyltransferase Inhibitor Protein 71; Constitutive Heat Shock Protein 70; Epididymis Luminal Protein 33; Heat Shock Cognate Protein 54; Heat Shock 70 KDa Protein 8; Heat Shock 70kd Protein 10; Heat Shock 70kD Protein 8; EC 3.6.4.10; HEL-S-72p; HEL-33; HSC54; HSP71; NIP71; LAP1
About Gene Symbol
HSPA8 (Heat Shock Protein Family A (Hsp70) Member 8) is a molecular chaperone involved in cellular metabolism. It is an important research target in metabolic disease, cancer metabolism, and drug discovery and is widely studied to elucidate cellular mecha
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