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KD-Validated Anti-DNA ligase 4 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1246
Application
WB, FC, ICC
Uniprot Id
P49917
Reactivity
Human
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-DNA ligase 4 antibody (Cat#ABG1246). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-DNA ligase 4 antibody (Cat#ABG1246, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-DNA ligase 4 antibody (Cat#ABG1246). DNA ligase 4 expression in wild type (WT) and DNA ligase 4 shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-DNA ligase 4 antibody (Cat#ABG1246, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of DNA ligase 4 expression in HeLa cells using DNA ligase 4 antibody (Cat#ABG1246, 1:2,000). Green, isotype control; red, DNA ligase 4.
  • Immunocytochemical staining of Hela cells with DNA ligase 4 antibody (Cat#ABG1246, 1:1,000). Nuclei were stained blue with DAPI; DNA ligase 4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1246-100 3 days 100 µL $499.00
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ABG1246-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-DNA ligase 4 Rabbit Monoclonal Antibody
Accession (Primary)P49917
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names23GB 2515
Immunogen
A synthesized peptide derived from human DNA Ligase IV
Species Reactivity
Reacts WithHuman
Calculated MW
Predicted, 104 kDa , observed, 103 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000

Target Information: ( P49917 )

Gene NameLIG4
Other Gene Names
LIG4; DNA Ligase 4; Polydeoxyribonucleotide Synthase [ATP] 4; DNA Ligase IV; Ligase IV, DNA, ATP-Dependent; Polynucleotide Ligase; DNA Repair Enzyme; DNA Joinase; EC 6.5.1.1; Sealase; LIG4S
About Gene Symbol
LIG4 (DNA Ligase 4) is a biosynthetic enzyme involved in DNA damage response and repair. It is an important research target in metabolic disease, cancer metabolism, and drug discovery and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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