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KD-Validated Anti-MCM3 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1256
Application
WB, ICC
Uniprot Id
P25205
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-MCM3 antibody (Cat#ABG1256). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-MCM3 antibody (Cat#ABG1256, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-MCM3 antibody (Cat#ABG1256). MCM3 expression in wild type (WT) and MCM3 shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-MCM3 antibody (Cat#ABG1256, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Immunocytochemical staining of HepG2 cells with MCM3 antibody (Cat#ABG1256, 1:1,000). Nuclei were stained blue with DAPI; MCM3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1256-100 3 days 100 µL $499.00
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ABG1256-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-MCM3 Rabbit Monoclonal Antibody
Accession (Primary)P25205
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names23GB 2490
Immunogen
A synthesized peptide derived from human MCM3
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 91 kDa , observed, 100 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Immunocytochemistry (IC): 1:100-1:1,000

Target Information: ( P25205 )

Gene NameMCM3
Other Gene Names
MCM3; Minichromosome Maintenance Complex Component 3; DNA Polymerase Alpha Holoenzyme-Associated Protein P1; DNA Replication Licensing Factor MCM3; RLF Subunit Beta; P1-MCM3; P102; MCM3 Minichromosome Maintenance Deficient 3 (S. Cerevisiae); Minichromosome Maintenance Deficient (S. Cerevisiae) 3; MCM3 Minichromosome Maintenance Deficient 3; Replication Licensing Factor, Beta Subunit; Minichromosome Maintenance Deficient 3; Cervical Cancer Proto-Oncogene 5; DNA Replication Factor MCM3; HRlf Beta Subunit; EC 3.6.4.12; HCC5; P1.H; RLFB
About Gene Symbol
MCM3 (Minichromosome Maintenance Complex Component 3) is a proto-oncogene involved in gene transcription and regulation. It is an important research target in cancer research and is widely studied to elucidate cellular mechanisms and identify therapeutic
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