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KD-Validated Anti-MLKL Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1259
Application
WB, FC, ICC
Uniprot Id
Q8NB16
Reactivity
Human, Mouse
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-MLKL antibody (Cat#ABG1259). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-MLKL antibody (Cat#ABG1259, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-MLKL antibody (Cat#ABG1259). MLKL expression in wild type (WT) and MLKL shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-MLKL antibody (Cat#ABG1259, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of MLKL expression in HepG2 cells using MLKL antibody (Cat#ABG1259, 1:2,000). Green, isotype control; red, MLKL.
  • Immunocytochemical staining of HepG2 cells with MLKL antibody (Cat#ABG1259, 1:1,000). Nuclei were stained blue with DAPI; MLKL was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1259-100 3 days 100 µL $499.00
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ABG1259-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-MLKL Rabbit Monoclonal Antibody
Accession (Primary)Q8NB16
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names23GB 2895
Immunogen
A synthesized peptide derived from human MLKL
Species Reactivity
Reacts WithHuman, Mouse
Calculated MW
Predicted, 54 kDa , observed, 54 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000

Target Information: ( Q8NB16 )

Gene NameMLKL
Other Gene Names
Mixed Lineage Kinase Domain-Like Protein; FLJ34389; HMLKL; Mixed Lineage Kinase Domain-Like
About Gene Symbol
MLKL (Mixed Lineage Kinase Domain-Like Protein) is a protein kinase and signaling regulator involved in cell signaling and proliferation. It is an important research target in cancer, cell signaling, and therapeutic target research and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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