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KD-Validated Anti-Peptidase D Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1264
Application
WB, FC, ICC
Uniprot Id
P12955
Reactivity
Human
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-Peptidase D antibody (Cat#ABG1264). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Peptidase D antibody (Cat#ABG1264, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-Peptidase D antibody (Cat#ABG1264). Peptidase D expression in wild type (WT) and peptidase D shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Peptidase D antibody (Cat#ABG1264, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of Peptidase D expression in HT-1080 cells using Peptidase D antibody (Cat#ABG1264, 1:2,000). Green, isotype control; red, Peptidase D.
  • Immunocytochemical staining of HT-1080 cells with Peptidase D antibody (Cat#ABG1264, 1:1,000). Nuclei were stained blue with DAPI; Peptidase D was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1264-100 3 days 100 µL $499.00
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ABG1264-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-Peptidase D Rabbit Monoclonal Antibody
Accession (Primary)P12955
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names23GB 2830
Immunogen
A synthesized peptide derived from human PRD
Species Reactivity
Reacts WithHuman
Calculated MW
Predicted, 55 kDa , observed, 55 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000

Target Information: ( P12955 )

Gene NamePEPD
Other Gene Names
PEPD; Peptidase D; Xaa-Pro Dipeptidase; Imidodipeptidase; Proline Dipeptidase; X-Pro Dipeptidase; Prolidase; Testicular Tissue Protein Li 138; Aminoacyl-L-Proline Hydrolase; EC 3.4.13.9; PROLIDASE; PRD
About Gene Symbol
PEPD (Peptidase D) is a protease enzyme involved in cellular metabolism. It is an important research target in metabolic disease, cancer metabolism, and drug discovery and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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