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KD-Validated Anti-PFKM Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1265
Application
WB, FC, ICC
Uniprot Id
P08237
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-PFKM antibody (Cat#ABG1265). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PFKM antibody (Cat#ABG1265, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-PFKM antibody (Cat#ABG1265). PFKM expression in wild type (WT) and PFKM shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-PFKM antibody (Cat#ABG1265, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of PFKM expression in HepG2 cells using PFKM antibody (Cat#ABG1265, 1:2,000). Green, isotype control; red, PFKM.
  • Immunocytochemical staining of HepG2 cells with PFKM antibody (Cat#ABG1265, 1:1,000). Nuclei were stained blue with DAPI; PFKM was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1265-100 3 days 100 µL $499.00
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ABG1265-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-PFKM Rabbit Monoclonal Antibody
Accession (Primary)P08237
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names23GB2740
Immunogen
A synthesized peptide derived from human Fructose 6 Phosphate Kinase
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 85 kDa, observed, 75 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000

Target Information: ( P08237 )

Gene NamePFKM
Other Gene Names
PFKM; Phosphofructokinase, Muscle; PPP1R122; PFK-1; PFKX; ATP-Dependent 6-Phosphofructokinase, Muscle Type; Protein Phosphatase 1, Regulatory Subunit 122; Phosphofructokinase, Polypeptide X; Phosphofructo-1-Kinase Isozyme A; 6-Phosphofructokinase Type A; Phosphohexokinase; EC 2.7.1.11; ATP-PFK; PFK-A; 6-Phosphofructokinase, Muscle Type; 6-Phosphofructo-1-Kinase; Phosphofructokinase 1; Phosphofructokinase-M; EC 2.7.1; PFK-M; GSD7; PFK1; PFKA
About Gene Symbol
PFKM (Phosphofructokinase, Muscle) is a protein phosphatase involved in cell signaling and proliferation. It is an important research target in cancer, cell signaling, and therapeutic target research and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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