KD-Validated Anti-Phospholipase A2 activating protein Rabbit Monoclonal Antibody

Western blotting analysis using anti-Phospholipase A2 activating protein antibody (Cat#ABG1267). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Phospholipase A2 activating protein antibody (Cat#ABG1267, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.

Western blotting analysis using anti-Phospholipase A2 activating protein antibody (Cat#ABG1267). Phospholipase A2 activating protein expression in wild type (WT) and phospholipase A2 activating protein shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Phospholipase A2 activating protein antibody (Cat#ABG1267, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.

Flow cytometric analysis of Phospholipase A2 activating protein expression in HepG2 cells using Phospholipase A2 activating protein antibody (Cat#ABG1267, 1:2,000). Green, isotype control; red, Phospholipase A2 activating protein.

Immunocytochemical staining of HepG2 cells with Phospholipase A2 activating protein antibody (Cat#ABG1267, 1:1,000). Nuclei were stained blue with DAPI; Phospholipase A2 activating protein was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.