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KD-Validated Anti-AMPK alpha 1 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1270
Application
WB, FC, ICC
Uniprot Id
Q13131
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-AMPK alpha 1 antibody (Cat#ABG1270). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-AMPK alpha 1 antibody (Cat#ABG1270, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-AMPK alpha 1 antibody (Cat#ABG1270). AMPK alpha 1 expression in wild type (WT) and AMPK alpha 1 shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-AMPK alpha 1 antibody (Cat#ABG1270, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of AMPK alpha 1 expression in HepG2 cells using AMPK alpha 1 antibody (Cat#ABG1270, 1:2,000). Green, isotype control; red, AMPK alpha 1.
  • Immunocytochemical staining of HepG2 cells with AMPK alpha 1 antibody (Cat#ABG1270, 1:1,000). Nuclei were stained blue with DAPI; AMPK alpha 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1270-100 3 days 100 µL $499.00
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ABG1270-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-AMPK alpha 1 Rabbit Monoclonal Antibody
Accession (Primary)Q13131
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names23GB2685
Immunogen
A synthesized peptide derived from human AMPK alpha 1
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 64 kDa , observed, 63 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000

Target Information: ( Q13131 )

Gene NamePRKAA1
Other Gene Names
PRKAA1; Protein Kinase AMP-Activated Catalytic Subunit Alpha 1; 5'-AMP-Activated Protein Kinase Catalytic Subunit Alpha-1; Protein Kinase, AMP-Activated, Alpha 1 Catalytic Subunit; Hydroxymethylglutaryl-CoA Reductase Kinase; Acetyl-CoA Carboxylase Kinase; Tau-Protein Kinase PRKAA1; AMPK Subunit Alpha-1; AMPK, Alpha, 1; ACACA Kinase; HMGCR Kinase; EC 2.7.11.1; AMPKa1; 5'-AMP-Activated Protein Kinase, Catalytic Alpha-1 Chain; AMP-Activated Protein Kinase, Catalytic, Alpha-1; AMP -Activate Kinase Alpha 1 Subunit; AMPK Alpha 1; EC 2.7.11.27; EC 2.7.11.31; EC 2.7.11.26; EC 2.7.11; AMPKA1; AMPK1; AMPK
About Gene Symbol
PRKAA1 (Protein Kinase AMP-Activated Catalytic Subunit Alpha 1) is a protein kinase and signaling regulator involved in cell signaling and proliferation. It is an important research target in cancer, cell signaling, and therapeutic target research and is
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