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KD-Validated Anti-Tripartite Motif Containing 25 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1294
Application
WB, FC, ICC
Uniprot Id
Q14258
Reactivity
Human, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-TRIM25 antibody (Cat#ABG1294). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-TRIM25 antibody (Cat#ABG1294, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-TRIM25 antibody (Cat#ABG1294). TRIM25 expression in wild-type (WT) and TRIM25 knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-TRIM25 antibody (Cat#ABG1294, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of TRIM25 expression in HepG2 cells using anti-TRIM25 antibody (Cat#ABG1294, 1:2,000). Green, isotype control; red, TRIM25.
  • Immunocytochemical staining of HepG2 cells with anti-TRIM25 antibody (Cat#ABG1294, 1:1,000). Nuclei were stained blue with DAPI; TRIM25 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1294-100 3 days 100 µL $499.00
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ABG1294-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-Tripartite Motif Containing 25 Rabbit Monoclonal Antibody
Accession (Primary)Q14258
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names25GB2695
Immunogen
A synthesized peptide derived from human TRIM25
Species Reactivity
Reacts WithHuman, Rat
Calculated MW
Predicted, 71 kDa; observed, 71 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western blotting (WB): 1:1,000-1:5,000Flow Cytometry (FCM): 1:2,000Immunocytochemistry (IC): 1:100-1:1,000

Target Information: ( Q14258 )

Gene NameTRIM25
Other Gene Names
TRIM25; Tripartite Motif Containing 25; RNF147; EFP; ZNF147; Zinc Finger Protein 147 (Estrogen-Responsive Finger Protein); RING-Type E3 Ubiquitin Transferase TRIM25; Ubiquitin/ISG15-Conjugating Enzyme TRIM25; Tripartite Motif-Containing Protein 25; Estrogen-Responsive Finger Protein; E3 Ubiquitin/ISG15 Ligase TRIM25; RING Finger Protein 147; RING-Type E3 Ubiquitin Transferase; Tripartite Motif Protein TRIM25; Tripartite Motif-Containing 25; Zinc Finger Protein-147; Zinc Finger Protein 147; EC 6.3.2.N3; EC 2.3.2.27; Z147
About Gene Symbol
TRIM25 (Tripartite Motif Containing 25) is a ubiquitin E3 ligase involved in ubiquitin-proteasome pathway. It is an important research target in gene regulation, development, and cancer research and is widely studied to elucidate cellular mechanisms and i
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