KD-Validated Anti-TRNA Methyltransferase 2 Homolog Rabbit Monoclonal Antibody

Western blotting analysis using anti-tRNA methyltransferase 2 homolog A antibody (Cat#ABG1296). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-tRNA methyltransferase 2 homolog A antibody (Cat#ABG1296, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.

Western blotting analysis using anti-tRNA methyltransferase 2 homolog A antibody (Cat#ABG1296). tRNA methyltransferase 2 homolog A expression in wild type (WT) and tRNA methyltransferase 2 homolog A (TRMT2A) knockdown (KD) HSHC cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-tRNA methyltransferase 2 homolog A antibody (Cat#ABG1296, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.

Flow cytometric analysis of tRNA methyltransferase 2 homolog A expression in HepG2 cells using anti-tRNA methyltransferase 2 homolog A antibody (Cat#ABG1296, 1:2,000). Green, isotype control; red, tRNA methyltransferase 2 homolog A.

Immunocytochemical staining of HepG2 cells with anti-tRNA methyltransferase 2 homolog A antibody (Cat#ABG1296, 1:1,000). Nuclei were stained blue with DAPI; tRNA methyltransferase 2 homolog A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Meidum. Scale bar, 20 µm.