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KD-Validated Anti-Cysteinyl-TRNA Synthetase 1 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1297
Application
WB, FC, ICC
Uniprot Id
P49589
Reactivity
Human, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-cysteinyl-tRNA synthetase 1 antibody (Cat#ABG1297). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-cysteinyl-tRNA synthetase 1 antibody (Cat#ABG1297, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-cysteinyl-tRNA synthetase 1 antibody (Cat#ABG1297). Cysteinyl-tRNA synthetase 1 expression in wild-type (WT) and cysteinyl-tRNA synthetase 1 (CARS1) knockdown (KD) HSHC cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-cysteinyl-tRNA synthetase 1 antibody (Cat#ABG1297, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of Cysteinyl-tRNA synthetase 1 expression in HT-1080 cells using anti-Cysteinyl-tRNA synthetase 1 antibody (Cat#ABG1297, 1:2,000). Green, isotype control; red, Cysteinyl-tRNA synthetase 1.
  • Immunocytochemical staining of HT-1080 cells with anti-Cysteinyl-tRNA synthetase 1 antibody (Cat#ABG1297, 1:1,000). Nuclei were stained blue with DAPI; Cysteinyl-tRNA synthetase 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1297-100 3 days 100 µL $499.00
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ABG1297-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-Cysteinyl-TRNA Synthetase 1 Rabbit Monoclonal Antibody
Accession (Primary)P49589
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names25GB2790
Immunogen
A synthesized peptide derived from human CARS
Species Reactivity
Reacts WithHuman, Rat
Calculated MW
Predicted, 85 kDa; observed, 75 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western blotting (WB): 1:1,000-1:5,000Flow Cytometry (FCM): 1:2,000Immunocytochemistry (IC): 1:100-1:1,000

Target Information: ( P49589 )

Gene NameCARS1
Other Gene Names
CARS1; Cysteinyl-TRNA Synthetase 1; CARS; Cysteine TRNA Ligase1,Cytoplasmic; Cysteine--TRNA Ligase, Cytoplasmic; Cysteinyl-TRNA Synthetase; EC 6.1.1.16; Cysteine Translase; MGC:11246; EC 6.1.1; MCDDBH; CYSRS; CysRS; MDBH
About Gene Symbol
CARS1 (Cysteinyl-TRNA Synthetase 1) is a biosynthetic enzyme involved in cellular metabolism. It is an important research target in metabolic disease, cancer metabolism, and drug discovery and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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