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KD-Validated Anti-AIFM1 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1299
Application
WB, FC, ICC
Uniprot Id
O95831
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-AIFM1 antibody (Cat#ABG1299). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-AIFM1 antibody (Cat#ABG1299, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-AIFM1 antibody (Cat#ABG1299). AIFM1 expression in wild-type (WT) and AIFM1 knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-AIFM1 antibody (Cat#ABG1299, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of AIFM1 expression in HepG2 cells using anti-AIFM1 antibody (Cat#ABG1299, 1:2,000). Green, isotype control; red, AIFM1.
  • Immunocytochemical staining of HepG2 cells with anti-AIFM1 antibody (Cat#ABG1299, 1:1,000). Nuclei were stained blue with DAPI; AIFM1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1299-100 3 days 100 µL $499.00
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ABG1299-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-AIFM1 Rabbit Monoclonal Antibody
Accession (Primary)O95831
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names25GB2810
Immunogen
A synthesized peptide derived from human AIF
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 67 kDa; observed, 67 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western blotting (WB): 1:1,000-1:5,000Flow Cytometry (FCM): 1:2,000Immunocytochemistry (IC): 1:100-1:1,000

Target Information: ( O95831 )

Gene NameAIFM1
Other Gene Names
AIFM1; Apoptosis Inducing Factor Mitochondria Associated 1; AIF; CMTX4; DFNX5; PDCD8; Apoptosis-Inducing Factor, Mitochondrion-Associated, 1; Programmed Cell Death 8 (Apoptosis-Inducing Factor); Apoptosis-Inducing Factor 1, Mitochondrial; Auditory Neuropathy, X-Linked Recessive 1; AUNX1; NAMSD; Neuropathy,Axonal, Motor-Sensory With Deafness And Mental Retardation (Cowchock Syndrome); Apoptosis Inducing Factor, Mitochondria Associated 1; Striatal Apoptosis-Inducing Factor; Testicular Secretory Protein Li 4; Programmed Cell Death Protein 8; EC 1.6.99.-; COXPD6; SEMDHL; CMT2D; COWCK; NADMR
About Gene Symbol
AIFM1 (Apoptosis Inducing Factor Mitochondria Associated 1) is a metabolic enzyme involved in apoptosis. It is an important research target in hearing loss and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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