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KD-Validated Anti-Protein Tyrosine Kinase 2 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1302
Application
WB, FC, ICC
Uniprot Id
Q05397
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-protein tyrosine kinase 2 antibody (Cat#ABG1302). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-protein tyrosine kinase 2 antibody (Cat#ABG1302, 1:5,000) and HRP-conjugated goat anti rabbit secondary antibody respectively.
  • Western blotting analysis using anti-protein tyrosine kinase 2 antibody (Cat#ABG1302). Protein tyrosine kinase 2 expression in wild-type (WT) and protein tyrosine kinase 2 (PTK2) knockdown (KD) 293T cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-protein tyrosine kinase 2 antibody (Cat#ABG1302, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of Protein tyrosine kinase 2 expression in C2C12 cells using anti-Protein tyrosine kinase 2 antibody (Cat#ABG1302, 1:2,000). Green, isotype control; red, Protein tyrosine kinase 2.
  • lmmunocytochemical staining of C2C12 cells with Protein tyrosine kinase 2 antibody (Cat#ABG1302, 1:1,000). Nuclei were stained blue with DAPl; Protein tyrosine kinase 2 was stained magenta with Alexa Fluor® 647. lmages were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1302-100 3 days 100 µL $499.00
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ABG1302-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-Protein Tyrosine Kinase 2 Rabbit Monoclonal Antibody
Accession (Primary)Q05397
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names25GB2855
Immunogen
A synthesized peptide derived from human FAK
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 119 kDa; observed, 119 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western blotting (WB): 1:1,000-1:5,000Flow Cytometry (FCM): 1:2,000Immunocytochemistry (IC): 1:100-1:1,000

Target Information: ( Q05397 )

Gene NamePTK2
Other Gene Names
PTK2; Protein Tyrosine Kinase 2; PPP1R7; FAK; FAK; FADK; Protein Phosphatase 1 Regulatory Subunit 71; Focal Adhesion Kinase-Related Nonkinase; PTK2 Protein Tyrosine Kinase 2; Focal Adhesion Kinase 1; EC 2.7.10.2; Pp125FAK; P125FAK; FADK 1; FRNK; Protein Phosphatase 1, Regulatory Subunit 71; FAK-Related Non-Kinase Polypeptide; Protein-Tyrosine Kinase 2; EC 2.7.10
About Gene Symbol
PTK2 (Protein Tyrosine Kinase 2) is a receptor tyrosine kinase involved in cell migration and invasion. It is an important research target in cancer, cell signaling, and therapeutic target research and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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