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KD-Validated Anti-FLI1 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1309
Application
WB, FC, ICC
Uniprot Id
Q01543
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-FLI1 antibody (Cat#ABG1309). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-FLI1 antibody (Cat#ABG1309, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-FLI1 antibody (Cat#ABG1309). FLI1 expression in wild-type (WT) and FLI1 knockdown (KD) HT-1080 cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-FLI1 antibody (Cat#ABG1309, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of FLI1 expression in HT-1080 cells using anti-FLI1 antibody (Cat#ABG1309, 1:2,000). Green, isotype control; red, FLI1.
  • Immunocytochemical staining of HT-1080 cells with anti-FLI1 antibody (Cat#ABG1309, 1:1,000). Nuclei were stained blue with DAPI; FLI1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1309-100 3 days 100 µL $499.00
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ABG1309-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-FLI1 Rabbit Monoclonal Antibody
Accession (Primary)Q01543
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names25GB2875
Immunogen
A synthesized peptide derived from human FLI1
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 51 kDa; observed, 51 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western blotting (WB): 1:1,000-1:5,000Flow Cytometry (FCM): 1:2,000Immunocytochemistry (IC): 1:100-1:1,000

Target Information: ( Q01543 )

Gene NameFLI1
Other Gene Names
FLI1; Fli-1 Proto-Oncogene, ETS Transcription Factor; SIC-; EWSR2; FLI-1; Friend Leukemia Integration 1 Transcription Factor; Friend Leukemia Virus Integration 1; Transcription Factor ERGB; Ewing Sarcoma Breakpoint Region 2; Proto-Oncogene Fli-1; BDPLT21
About Gene Symbol
FLI1 (Fli-1 Proto-Oncogene, ETS Transcription Factor) is a proto-oncogene involved in gene transcription and regulation. It is an important research target in cancer research and infectious disease and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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