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KD-Validated Anti-IKBKB Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1311
Application
WB, FC, ICC
Uniprot Id
O14920
Reactivity
Human, Mouse
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-IKBKB antibody (Cat#ABG1311). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-IKBKB antibody (Cat#ABG1311, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-IKBKB antibody (Cat#ABG1311). IKBKB expression in wild type (WT) and IKBKB knockdown (KD) HSHC cells with 20 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-IKBKB antibody (Cat#ABG1311, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of IKBKB expression in HepG2 cells using anti-IKBKB antibody (Cat#ABG1311, 1:2,000). Green, isotype control; red, IKBKB.
  • Immunocytochemical staining of HepG2 cells with anti-IKBK antibody (Cat#ABG1311, 1:1,000). Nuclei were stained blue with DAPI; IKBK was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1311-100 3 days 100 µL $499.00
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ABG1311-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-IKBKB Rabbit Monoclonal Antibody
Accession (Primary)O14920
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names25GB2895
Immunogen
A synthesized peptide derived from human IKK beta
Species Reactivity
Reacts WithHuman, Mouse
Calculated MW
Predicted, 87 kDa; observed, 87 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western blotting (WB): 1:1,000-1:5,000Flow Cytometry (FCM): 1:2,000Immunocytochemistry (IC): 1:100-1:1,000

Target Information: ( O14920 )

Gene NameIKBKB
Other Gene Names
IKBKB; Inhibitor Of Nuclear Factor Kappa B Kinase Subunit Beta; IKK-Beta; NFKBIKB; IKK2; IKKB; Inhibitor Of Kappa Light Polypeptide Gene Enhancer In B-Cells, Kinase Beta; Inhibitor Of Nuclear Factor Kappa-B Kinase Subunit Beta; Nuclear Factor NF-Kappa-B Inhibitor Kinase Beta; Serine/Threonine Protein Kinase IKBKB; I-Kappa-B-Kinase Beta; I-Kappa-B Kinase 2; EC 2.7.11.10; IKK-2; Nuclear Factor Kappa B Kinase Subunit Beta; EC 2.7.11.1; EC 2.7.11; IMD15A; IMD15B; IMD15; IKK-B; IkBKB
About Gene Symbol
IKBKB (Inhibitor Of Nuclear Factor Kappa B Kinase Subunit Beta) is a serine/threonine protein kinase involved in NF-κB signaling. It is an important research target in cancer, cell signaling, and therapeutic target research and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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